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Install, activate, and get help.

Use this page for system requirements, Windows setup, license activation, release notes, and contact.

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30-Day Free Trial • No Credit Card Required • Windows OS Only

System & Software Requirements.

Confirm the workstation meets these requirements before you install.

System

Processor

Intel Core i5 or equivalent

RAM

8 GB or more

Storage

500 MB free space

Display

1280x720 resolution or higher

Software

Operating System

Windows 10 or Windows 11

PDF Reader

Adobe Acrobat Reader (required for PDF reports)

Microsoft Excel

Optional. Useful for editing Excel or CSV files; not required to run ToxGenie.

Microsoft Word

Optional. Useful for opening and editing .docx reports; not required to run ToxGenie.

Installation Guide for Windows.

 Windows Defender SmartScreen Warning

STEP 1

STEP 2

  • Extract the .zip.
  • Run the .exe.
  • Follow the on-screen steps to finish installation.
ToxGenie Support: Windows SmartScreen during installation

Activating Your Annual Subscription.

These steps are for Windows.

Step 1: Check Your Trial

  • Before the trial ends, open About in ToxGenie and note the expiry date.

Step 2: Purchase

  • Click Subscribe Now. Payment is processed by Lemon Squeezy.

Step 3: License Key

  • After payment, a license key is sent to your email. That key activates the full version for one year.

Step 4: Activate (online)

  1. Start ToxGenie.
  2. Open Help on the top menu.
  3. Choose Install License.
  4. Paste the key from the email (preferred) or type it.
  5. Click Submit. ToxGenie verifies the subscription and unlocks Academic or Professional features.

Step 5: Offline (institutional firewall)

If the PC cannot reach the license server, use Offline Mode in the same Install License window. Check Offline Mode, enter the key, and click Submit. Follow the on-screen request/response steps. Campus or company emails may be required.

ToxGenie Support: License activation preocess during installation
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Release Notes & Update History.

Updates for compliant, clearer toxicity data analysis.

1.3.8 [Current] - November 1, 2026

Overview
This release adds the OECD TG 202 Daphnia and OECD TG 203 Fish acute toxicity tests. It reorganises Create Data Sheet and Endpoint Analysis by OECD Test Guideline. It also fixes a license check that could ask for the license key again after an update.

New Features

  • OECD TG 202, Daphnia sp. Acute Immobilisation Test: 
    • The data sheet records immobilised daphnids at 24 and 48 hours.
    • The EC50 is estimated at each time by Probit (Maximum Likelihood), Logit, Spearman-Karber, Trimmed Spearman-Karber, Moving Average-Angle, or Graphical Interpolation.
    • Limit Test and Definitive Test are supported.
    • Control validity (10 percent immobilisation or less) is judged at the end of the test.
    • Abbott’s correction is not applied.
    • PDF and MS Word use the same sentences, tables, and figures.
  • OECD TG 203, Fish, Acute Toxicity Test: 
    • The data sheet records cumulative mortality on the OECD TG 203 observation schedule.
    • The LC50 is estimated at 24, 48, 72, and 96 hours.
    • Limit Test: below 50 percent mortality the report gives Point Estimates; at or above 50 percent it states that a Definitive Test is required.
    • Control mortality must not exceed 10 percent.
    • PDF and MS Word use the same sentences, tables, and figures.

Feature Improvements

  • Solvent control: 
    • When a solvent control is recorded, the EC50 or LC50 uses it as the baseline, and the report explains why.
    • Each recorded control has its own validity section. The two controls are not combined.
  • Algae Growth Inhibition Test: 
    • The confirmation before analysis shows the official OECD test name.
    • A non-standard exposure period keeps the table note and is not judged as met or not met.
  • Report tables: 
    • PDF and MS Word tables size each column to its text.
    • A wide table is split into parts with nearly equal numbers of columns.
    • Bold column titles stay on one line.
  • A proportion is reported to 5 decimal places.
  • An empty required Study Information field now always shows a notice, including when Estimation is clicked.
  • The data type list in the spreadsheet uses plain words. “Text or number, as typed” stays the default, so every value is kept exactly as entered.
  • Dialogs and notices open fully drawn, with the ToxGenie icon and no flicker. Each notice is sized to its text.

Bug Fixes

  • After an update, ToxGenie could ask for the license key again when Windows reported a different network adapter, for example after a Bluetooth, VPN, or Wi-Fi change.
    • The license is now tied to the Windows machine ID.
    • An existing license is moved automatically, with the same end date and license type.
  • The license end date is now checked against one time basis (UTC) for online and offline checks.
  • Deleting a column while a sheet was loading could overwrite the Dose header.
  • In the column-name editor, the arrow keys now move the text cursor inside the name.
  • An empty row in the OECD TG 202 or TG 203 sheet is no longer counted as a dose group.

Overview
This release adds Regression and Descriptive Statistics, lets the analyst choose the ICx method in Point Estimation, and marks a statistic that cannot be calculated.

New Features

  • Regression: Linear and nonlinear regression of each selected endpoint on Dose is on the Statistical Analysis menu and toolbar. Only Quantitative/Continuous Data is analyzed. Simple Regression writes one report for each endpoint. Combined Regression writes one report. Curves that share a scale are drawn together, and a curve on a different scale is drawn separately. PDF and MS Word use the same sentences, tables, and figures.
  • Descriptive Statistics: Dose-group summaries are on the Statistical Analysis menu and toolbar, before the other statistical methods. Count, Quantitative/Continuous, Quantal, and Ordinal Data can be summarised for the selected doses and endpoints. No significance test, correlation, regression, or endpoint is calculated. A statistic that the data cannot support is marked NC and explained in a note. PDF and MS Word use the same sentences and tables.

Feature Improvements

  • In Point Estimation, Count Data and Quantitative/Continuous Data ask the analyst to select Linear Interpolation or a Nonlinear Model for each variable. PDF and MS Word use that choice. Closing the window skips that variable.
  • Measurement Unit opens as Not Applicable in Endpoint Analysis and Statistical Analysis, whichever Toxicology Area is selected. A value loaded from the last saved input is kept.
  • A statistic that cannot be calculated is printed as NC, with the note “NC: Not Calculable.” It is not left blank and it is not printed as zero. A blank cell that the user left blank stays blank.

Bug Fixes

  • An update download that is interrupted can resume.
  • A character that is not English is not accepted. A notice explains that English is used so the study record can be mutually accepted. The program does not stop.

Overview
This release adds Correlation, records a later data change in the PDF and MS Word reports, and revises the main window.

New Features

  • Correlation: Pearson, Spearman, and Kendall tau-b are on the Statistical Analysis menu and toolbar. Count, Quantitative/Continuous, and Ordinal Data can be analyzed. Quantal Data is not analyzed. The dose-adjusted correlation is the primary result. PDF and MS Word use the same sentences, tables, and figures.
  • Data Amendment Record: After the first analysis of a sheet, a later edit is kept and is printed under the raw-data table. PDF and MS Word use the same sentences. The first entry of a new sheet is not printed. If no reason is entered, the report prints “not recorded”. The edited value is still used.

Feature Improvements

  • The main menu is a row of names. The selected menu opens its toolbar beside Spreadsheet Setting.
  • The open data sheet tab is shown in blue. Right-click a sheet tab to save that sheet or close it.
  • Cell, clipboard, and row or column actions sit on their own row under Spreadsheet Setting. While a value is being typed, each arrow key keeps that value and moves one cell.

Bug Fixes

  • The notice shown when an analysis opens is drawn once.
  • An edit made while the amendment reason window is open is still recorded.
  • If the Data Amendment Record cannot be written, the report is still created.

Overview
This release adds the Government license, completes the Two-Way ANOVA report in PDF and MS Word, and keeps the Study No. and graph labels as entered.

New Features

  • Government license: This license includes watermarks and headers on PDF and MS Word reports. The GLP Validation Kit is not included. The kit remains in the Professional license only. A key can also be entered when the computer cannot reach the license server.
  • Two-Way ANOVA: The MS Word report is now available. PDF and MS Word use the same sentences, tables, and order. Descriptive statistics show N, Mean, SD, SE, CV (%), and Median.

Feature Improvements

  • The Statistical Analysis menu and toolbar follow one order: Control Chart; Two Group Comparisons and Multiple Group Comparisons; Frequency Analysis; dose-response trend tests; Two-Way ANOVA and Repeated Measures ANOVA. The names are unchanged.
  • A Study No. such as 00123 stays 00123 in the report. PDF and MS Word both keep it.
  • A long Y-axis title stays on the figure. If the unit is already in the measurement name, it is not added again.
  •  

Bug Fixes

  • If Study Information is incomplete, ToxGenie does not create a report and does not open the previous report.
  • An update is installed only when the downloaded file contains the ToxGenie installer. If a study is open, ToxGenie asks you to save it first. The update waits until ToxGenie has closed..

Overview
This release improves the data-entry screens and corrects errors in the data-input GUI.

Feature Improvements

  • Data-entry GUI: The data-input screens are clearer and easier to use.

Bug Fixes

  • Data input: Errors in the data-entry screens are corrected.

Overview
This release makes analysis and data-sheet windows open more steadily, keeps PDF and Word reports aligned, and analyzes the measurement column you select.

New Features

  • Point Estimation: When at least two partial responses are seen and the response increases with dose, you choose Probit or Logit. PDF and MS Word both use that choice.
  • Control Chart: All studies with a number are included in the limits and the chart. The MS Word report uses the same sentences as the PDF.

Feature Improvements

  • Analysis and data-sheet windows open after the screen is ready.
  • PDF and MS Word place one sentence on each line. The words are unchanged. Text entered in Study Information stays as typed.
  • Hypothesis Tests analyze the measurement column you select, and both reports name the same test.
  • A number such as 1.0 stays 1.0 after you save the sheet as Excel and open it again.

Bug Fixes

  • Analysis and data-sheet windows no longer jump or flash.

Overview
This release changes how updates are installed. If you are using 1.3.0 or 1.3.1, install 1.3.2 from this website. Do not wait for the in-app auto-update alone. On those versions the update file may download without an install window.

Feature Improvements

  • After the update file is ready, ToxGenie asks whether to install now. Yes closes ToxGenie, installs the update, and restarts. This includes the check at startup.
  • Help → Check for Updates… also installs the update. If a check is already running, wait until it finishes, then choose the same menu again.

Overview
This release aligns hypothesis-testing defaults with U.S. EPA practice for small replicate counts, matches PDF and Word wording, and unifies on-screen guidance.

New Features

  • Clustered Quantal Trend: When Quantal Data are recorded by replicate vessel (tank or chamber), Dose–Response Trend Analysis can use the Rao–Scott Cochran–Armitage test instead of the standard Cochran–Armitage test.
  • NOEC/LOEC or NOAEL/LOAEL: Hypothesis Tests use the label that matches the study type. Algae growth inhibition reports use NOEC/LOEC only.
  • Each report has Analyst and Signature, then Approved by ( ): for a handwritten name and role (Study Director, advisor, or reviewer). This is not an electronic signature.

Feature Improvements

  • Shapiro–Wilk and Levene tests now use α = 0.01, as in U.S. EPA for studies with few replicates. The main comparison test remains α = 0.05.
  • The MS Word report uses the same wording, tables, and notes as the finalized PDF.
  • Numbers and labels you enter are shown in the report as recorded.
  • Column selection, data-type checks, and related messages use one card layout across analysis modules.

Overview
This release redesigns the interface, adds trend and repeated-measures methods, separates original study files from session files, and switches licensing to Lemon Squeezy.

New Features

  • Trend-based multiple-group comparisons versus control, using Williams’ test or Shirley’s test according to the data distribution.
  • Repeated Measures ANOVA for the same subjects over time, with automated post-hoc pairwise comparisons (Dunnett’s, Steel’s, Student’s t-test, or Mann–Whitney).
  • Reports include a Summary section.
  • After Estimation, ToxGenie remembers the folder used for PDF or Word reports and uses it for the next analysis.
  • Lemon Squeezy issues the license key after subscription. Keys and subscriptions are managed in Lemon Squeezy.
  • Create Data Sheet includes ordinal templates for histopathology severity, clinical signs, and FOB scores. These paths do not force parametric assumption tests.
  • Data Sheet Utility → Convert to ToxGenie Data builds a ToxGenie-ready sheet from existing tables after confirmation. Original cell values are not changed. A new sheet is created.

Feature Improvements

  • The graphical user interface is redesigned.
  • Analysis PDFs are generated without password protection. Archive them according to facility SOPs.
  • File → Save As ToxGenie stores the working session as a .tox file. It is not a substitute for the original Excel or CSV study file. Reopen it with File → Open ToxGenie.
  • Double-clicking a .tox file in Windows Explorer does not start ToxGenie. Open session files from the File menu.

Overview
This release adds the GLP Validation Kit documents and protocols, and watermarking for PDF and Word reports.

New Features

  • Declaration of Conformity: system reliability and software compliance based on GAMP 5 Category 3.
  • Part 11 White Paper: hybrid architecture for data integrity (21 CFR Part 11 / Annex 11) without a required database server.
  • IQ Protocol (Installation Qualification): scans workstation specifications and includes required manual verification checkpoints.
  • OQ Protocol: one-click run of three regulatory-aligned toxicity datasets (Sperling-Karber [SK], hypothesis testing, and algae growth inhibition), PDF output, and instructions to check results against EPA/OECD reference values.
  • Custom watermarks on exported PDF and MS Word (.docx) reports.

Overview
This release adds quantal hypothesis tests, automatic model choice for point estimation, and Word export alongside PDF.

New Features

  • Hypothesis testing for quantal (binary) toxicity data.
  • Point estimation with linear and non-linear regression. ToxGenie selects the best-fit model for LC/EC estimation.
  • Export to MS Word (.docx) in addition to PDF. Word files are editable for GLP reports or manuscripts.
  • Progress indicator during report generation.
  • Copy and paste from Microsoft Excel into the ToxGenie Data Sheet, keeping structure.

Feature Improvements

  • Faster processing of large toxicity datasets.
  • Data Sheet entry and editing.
  • Update system.

Overview
This release simplifies Create Data, adds clipboard support, and shows 95% confidence intervals on result charts.

New Features

  • Create Data workflow for experimental parameters.
  • Copy and paste in the Data Sheet, including transfer from Microsoft Excel.
  • Analysis charts include 95% confidence interval overlays for LC50/EC50 estimates.

Feature Improvements

  • NOEC and LOEC decision-tree logic for non-monotonic dose–response data.

Overview
This release adds the first NOEC/LOEC estimation path and tightens file import.

New Features

  • Foundational logic for NOEC/LOEC effect-concentration estimation.

Feature Improvements

  • 95% confidence intervals for acute toxicity endpoints.

Bug Fixes

  • UI errors when switching between data sheets.
  • Parser for .xlsx and .csv files to prevent data loss during import.

Overview
First release of ToxGenie for Windows. The engine calculates LC50, EC50, and NOEC/LOEC for OECD, U.S. EPA, EU-REACH, and K-REACH study workflows.

New Features

  • LC50, EC50, and NOEC/LOEC calculation.
  • Alignment with OECD, U.S. EPA, EU-REACH, and K-REACH testing guidelines.
  • Three-step workflow: Input → Analyze → Report.
  • PDF reporting for regulatory submissions.
  • Original Excel or CSV study files remain the study record.

Frequently Asked Questions

Can I use ToxGenie on a Mac (macOS)?
 Q Q

ToxGenie is built for Windows 10/11. Mac users may run it on a virtual machine such as Parallels.

Install Adobe Acrobat Reader. It is required to generate and open ToxGenie PDF reports.

No. Each license key is bound to a user and machine. For lab or institution licenses, email toxgenie@toxgenie.com.

Master’s and Ph.D. students writing a thesis may receive a complimentary 1-year license. A second year is granted if, before the first year ends, you email us that the thesis has been submitted or is in preparation. Maximum: 2 years. Apply from your university email; see Pricing.

Install ToxGenie on the new PC, then email toxgenie@toxgenie.com for a new license code.

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