Discover ToxGenie Data Analysis Methods
ToxGenie empowers toxicologists and ecotoxicologists to create professional reports in minutes, no stats expertise needed.
Simply input data, select your analysis, and generate regulatory compliant results with one click.
1. Flowchart for Statistical Analysis
- ToxGenie offer a full suite of statistical methods for Point Estimation and Hypothesis Test.
- ToxGenie automatically recommends appropriate statistical methods for LC50, EC50, LD50, NOEC, and LOEC estimation based on predefined decision-making rules.
2. Entering Your Test Data
- Since ToxGenie is specialized for toxicological data analysis, you need to input data in a specified format. You can input your toxicological test data in two ways.
- Using the Create Data Sheet menu to input data directly. or
- Modifying an Excel file in the Sample Data folder, saving it as .xlsx or .csv.
- Importing it via the File menu.
3. Determination of Definitive Test Concentrations
- Automatically recommend optimal concentrations for acute and chronic toxicity tests using preliminary test data.
Example Workflow: Determining Chronic Definitive Test Concentrations in 3 Simple Steps
Enter Your Data
- Select Create Data Sheet tab.
- Select Range-Finding Test Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Definitive Study Concentration Setting in the drop-down menu.
- Enter or select all information related to the test.
- Select Chronic Toxicity in the Study Title.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
4. LC50, EC50, and LD50 Estimation
- Calculate LC50, EC50, LD50 with 95% confidence intervals for acute and chronic toxicity tests.
- The optimized analysis method is suggested based on the characteristics of the input data.
Supported Point Estimation Analysis Methods for LC50, EC50, LD50 Estimation
- Moving Average-Angle Method
- Spearman-Karber Method
- Trimmed Spearman-Karber Method
- Probit Method
- Logit Method
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantal Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Point Estimation in the drop-down menu.
- Select the appropriate Analysis Method tab for your data.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
5. IC25 and IC50 Analysis
- Estimate IC25 and IC50 with 95% confidence intervals for ecotoxicity and cytotoxicity tests by automatically selecting the best-fit model from five linear and non-linear regression models based on your data.
- Also, it can be used to calculate EC25 and EC50 with 95% confidence intervals for quantitative data (e.g., reproduction, growth, etc.).
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the IC25 and IC50.
Supported Linear and Non-Linear Models for IC25 and IC50 Estimation
- Exponential Model
- Gompertz Model
- Hormesis Model
- Linear Model
- Logistic Model
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Point Estimation in the drop-down menu.
- Select the Inhibiting Concentration (ICp) Tab.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
6. Algae and Cyanobacteria, Growth Inhibition Test
- Provide a comprehensive analysis for test validity, EC10, EC20, EC50, NOEC, and LOEC for both average growth rate and yield, based on OECD TG 201.
- The appropriate statistical analysis procedures and methods are automatically executed based on the characteristics of the input data
Estimation of EC10, EC20, EC50, NOEC, and LOEC based on both Average Growth Rate and Yield
- Test Validity Calculation in the Limit or the Definitive Test
- Estimation of EC10, EC20, EC50, and their associated 95% Confidence Intervals using Non-Linear Regression
- Estimation of NOEC and LOEC according to the Flowchart for Statistical Analysis presented above
- Growth Curve (Plot of Biomass versus Time)
Enter Your Data
- Select Create Data Sheet tab.
- Select Algae and Cyanobacteria, Growth Inhibition Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Algae and Cyanobacteria, Growth Inhibition Test in the drop-down menu.
- Be sure to select either the Limit Test or the Definitive Test in the test type.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
7. Estimation of NOEC and LOEC for a Single Observation
- Provide a comprehensive analysis for a single endpoint, determining the NOEC and LOEC for both acute and chronic toxicity studies.
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the NOEC and LOEC.
- The appropriate statistical analysis procedures and methods are automatically executed based on the characteristics of the input data
For NOEC and LOEC Calculation
- Normality Test
- Homogeneity Test
- Data Transformation
- Statistical method selection follows the Flowchart for Statistical Analysis presented above
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data for a Single Observation in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Hypothesis Test for a Single Observation in the drop-down menu.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
8. Estimation of NOEC and LOEC for Multiple Observations
- Conducts comprehensive one-stop analysis for multiple endpoints across three or more test groups in studies such as Daphnia Reproduction and fish Early Life Stage (ELS) tests.
- The system automatically selects appropriate parametric or non-parametric statistical methods to estimate NOEC and LOEC.
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the NOEC and LOEC.
- The appropriate statistical analysis procedures and methods are automatically executed based on the characteristics of the input data
For NOEC and LOEC Calculation
- Normality Test
- Homogeneity Test
- Data Transformation
- Statistical method selection follows the Flowchart for Statistical Analysis presented above
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data for Multiple Observations in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Hypothesis Test for Multiple Observations in the drop-down menu.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
