Easy Toxicity Data Analysis Guide
Struggling with time-consuming toxicity data analysis? See how ToxGenie simplifies it.
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1. Flowchart for Toxicity Data Statistical Analysis
- ToxGenie offer a full suite of statistical methods for toxicity data analysis, including Point Estimation and Hypothesis Tests.
- ToxGenie automatically recommends appropriate statistical methods for LC50, EC50, LD50, NOEC, and LOEC estimation based on predefined decision-making rules.
2. Entering Your Test Data
- Since ToxGenie is specialized for toxicity data statistical analysis, you need to input data in a specified format. You can input your toxicity test data in two ways (Please see the figure below.).
- Using the Create Data Sheet menu to input data directly.
- Modifying an Excel file in the Sample Data folder, saving it as .xlsx or .csv.
- Importing it via the File menu.
3. Determination of Definitive Test Concentrations
- Automatically recommend optimal concentrations for acute and chronic toxicity tests using range-finding test data.
Example Workflow: Determining Chronic Definitive Test Concentrations in 3 Simple Steps
Enter Your Data
- Select Create Data Sheet tab.
- Select Range-Finding Test Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Definitive Study Concentration Setting in the drop-down menu.
- Enter or select all information related to the test.
- Select Chronic Toxicity in the Study Title.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
4. LC50, EC50, and LD50 Calculation
LC50: The concentration of a substance that is lethal to 50% of a test population, typically aquatic organisms, within a specified time.
EC50: The concentration of a substance that causes a specific effect in 50% of a test population, often related to non-lethal responses like immobility and growth inhibition.
LD50: The dose of a substance that causes death in 50% of a test population, usually expressed as mg/kg body weight.
- Calculate LC50, EC50, LD50 with 95% confidence intervals for acute and chronic toxicity tests.
- The optimized analysis method is suggested based on the characteristics of the input data.
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantal Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Point Estimation in the drop-down menu.
- Select the appropriate Analysis Method tab for your data.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
5. IC25 and IC50 Analysis
ICp is the inhibiting concentration for a (specified) percent effect. It represents a point estimate of a concentration of test material that is estimated to cause a designated percent impairment in a quantitative biological function, such as the size attained by organisms during a growth period.
For example, IC25 is the concentration estimated to result in organisms having a dry weight 25% lower than that attained by control organisms.
- Estimate IC25 and IC50 with 95% confidence intervals for ecotoxicity and cytotoxicity tests by automatically selecting the best-fit model from five linear and non-linear regression models based on your data.
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the IC25 and IC50.
Supported Linear and Non-Linear Models for IC25 and IC50 Estimation
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Point Estimation in the drop-down menu.
- Select the Inhibiting Concentration (ICp) Tab.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
6. Algae and Cyanobacteria, Growth Inhibition Test
- Provide a comprehensive analysis for test validity, EC10, EC20, EC50, NOEC, and LOEC for both average growth rate and yield, based on OECD TG 201.
- The appropriate statistical analysis procedures and methods are automatically executed based on the characteristics of the input data.
Estimation of EC10, EC20, EC50, NOEC, and LOEC based on both Average Growth Rate and Yield, with 95% confidence limits provided for EC50, automatically analyzed
- Test Validity Calculation in the Limit or the Definitive Test
- Estimation of EC10, EC20, EC50, and their associated 95% Confidence Intervals using Non-Linear Regression
- Estimation of NOEC and LOEC (Hypothesis Tests) according to the Flowchart for Statistical Analysis presented above
- Growth Curve (Plot of Biomass versus Time)
Enter Your Data
- Select Create Data Sheet tab.
- Select Algae and Cyanobacteria, Growth Inhibition Data in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Algae and Cyanobacteria, Growth Inhibition Test in the drop-down menu.
- Be sure to select either the Limit Test or the Definitive Test in the test type.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
Click here to view the detailed report.
7. Estimation of NOEC and LOEC for a Single Observation
- NOEC (No Observed Effect Concentration): The highest concentration of a test substance at which no statistically significant adverse effect is observed on the test organisms compared to the control group under defined experimental conditions.
- LOEC (Lowest Observed Effect Concentration): The lowest concentration of a test substance at which a statistically significant adverse effect is observed on the test organisms compared to the control group under defined experimental conditions.
Comprehensive toxicity data analysis for a single endpoint (For Example: OECD Test Guideline No. 209: Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation), to determine the NOEC and LOEC for both acute and chronic toxicity studies.
The method for calculating NOEC and LOEC is referred to as Hypothesis Testing.
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the NOEC and LOEC.
To understand why a minimum of three replicates is required for toxicity test data to calculate NOEC and LOEC in ecotoxicity testing, and to explore detailed analysis methods, visit my blog.
ToxGenie automatically performs all the major steps below to estimate NOEC and LOEC based on the characteristics of the input data.
Major Steps Required to Estimate NOEC and LOEC in Ecotoxicity Testing
- Normality Test
- Homogeneity Test
- Data Transformation when necessary
- Statistical method selection follows the Flowchart for Statistical Analysis presented above
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data for a Single Observation in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Hypothesis Test for a Single Observation in the drop-down menu.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
- Click here to view the detailed report.
8. Estimation of NOEC and LOEC for Multiple Observations
- NOEC (No Observed Effect Concentration): The highest concentration of a test substance at which no statistically significant adverse effect is observed on the test organisms compared to the control group under defined experimental conditions.
- LOEC (Lowest Observed Effect Concentration): The lowest concentration of a test substance at which a statistically significant adverse effect is observed on the test organisms compared to the control group under defined experimental conditions.
- Simultaneously analyzes multiple endpoints in studies such as Daphnids reproduction test and Fish Early Life Stage (ELS) test, providing NOEC and LOEC values for each endpoint.
- The system automatically selects appropriate parametric or non-parametric statistical methods to estimate NOEC and LOEC.
- A minimum of three replicates is necessary in a toxicity test to enable the calculation of the NOEC and LOEC.
- The appropriate statistical analysis procedures and methods are automatically executed based on the characteristics of the input data
Major Steps Required to Estimate NOEC and LOEC in Ecotoxicity Testing
- Normality Test
- Homogeneity Test
- Data Transformation
- Statistical method selection follows the Flowchart for Statistical Analysis presented above
Enter Your Data
- Select Create Data Sheet tab.
- Select Quantitative Data for Multiple Observations in the drop-down menu.
- Please refer to the Entering Your Test Data section above.
Run Estimation
- Select Analyze tab.
- Select Hypothesis Test for Multiple Observations in the drop-down menu.
- Enter or select all information related to the test.
- Click estimation button.
Generate Report Automatically
- Print or Save As the PDF report.
- Click here to view the detailed report.
